Figure 5.
MPs isolated from human atherosclerotic plaque activate the release of TNF and TNFR-1 from the human cell line ECV-304TNF. ECV-304TNF cells were incubated with MPs as described in Materials and Methods. A: Release of TNF. Values are mean ± SD (n = 6, each performed in duplicate). Significance of MP and TMI-2 effects was calculated by t-test (Mann-Whitney U-test). B: Dose-dependent effect of MPs (expressed as Annexin V+/μl) on TNF release. C: Release of TNFR-1 measured on the same samples as in A.