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. 2007 Nov;171(5):1713–1723. doi: 10.2353/ajpath.2007.070021

Figure 6.

Figure 6

Plaque MPs do not induce the release of exosome-associated full-length TNFR-1 and TNF from endothelial cells. HUVECs (A) or ECV-304TNF cells (B) were incubated with or without plaque MPs as indicated in Figures 4 and 5, respectively. The culture medium was centrifuged at 20,500 × g (45 minutes at 4°C) to pellet MPs, and half of the resulting supernatant (Sn 20,500 × g) was further centrifuged at 170,000 × g (16 hours at 4°C) to pellet exosomes and the other half left for the same time at 4°C. The two supernatants (Sn 20,500 × g; Sn 170,000 × g) and the 170,000 × g pellet were assayed for TNFR-1 (expressed as total amount, pg). For both cellular types, at least 98% of TNFR-1 or TNF was recovered in the 170,000 × g supernatant (Sn). Values are mean ± SD; n = 3. C: The culture medium of ECV-304TNF cells exposed to plaque MPs (Sn) contain only the cleaved form of TNF compared with 10 μg of recombinant human TNF (rTNF) on the left lane. Representative of two different samples analyzed.