Figure 2.

Specific DAB deposition in Golgi cisternae after DAB incubation of fixed or living ManII-HRP–expressing HeLa cells. HeLa ManII-HRP cells were either fixed and stained with DAB/H₂O₂ (a) or incubated alive with DAB/H₂O₂ and then fixed (b) before being processed for EM. As a control (c), wild-type HeLa cells were treated as in a. Note that the DAB precipitate was similarly restricted to Golgi cisternae in cells stained fixed or alive. Bar, 250 nm.