Figure 8.

Detection of GM10 by immunoelectron microscopic observation of cells after Golgi cross-linking. ManII-HRP HeLa cells cross-linked by DAB+H₂O₂ and allowed to recover for 120 min (a) or control ManII-HRP HeLa cells treated by H₂O₂ alone (b) were processed for cryoelectron microscopy and immunogold labeling using an anti-GM130 antibody (some gold particles are indicated by an arrow). In cross-linked cells, GM130 is detected on membrane structures, apposed to the inactivated cisternae, that do not display the normal shape of a Golgi complex. This is in marked contrast with the Golgi localization of GM130 observed in non–cross-linked HeLa cells (b). Bar, 500 nm.