Table 1.

Cilia length (micrometers) in wild-type and fleer mutant larvae depends on position in the pronephros

	Anterior	Midsegment	Posterior
wt
14 somites	1.3 ± 0.4 (n = 28)	1.7 ± 0.3 (n = 34)	1.1 ± 0.2 (n = 11)
48 hpf	11.5 ± 1.7 (n = 34)	8.5 ± 1.8 (n = 27)	5.5 ± 1 (n = 15)
Fleer−/−
14 somites	1.5 ± 0.3 (n = 68)	2.0 ± 0.5 (n = 52)	1.0 ± 0.2 (n = 18)
48 hpf	7.2 ± 1.6 (n = 115)	6.6 ± 1.3 (n = 79)	3.5 ± 1.3 (n = 18)

Cilia lengths were measured from confocal z-series projections (see Materials and Methods) by using ImageJ software. At early stages of development (14 hpf), cilia were similar in length in wild-type and flr embryos. At 48 hpf, cilia were consistently shorter in flr mutants. Data represents the mean cilia length ± SD; n equals the number of individual cilia measured. At least three different embryos were examined for each data point.