Figure 10.

Overexpression of LARGE restores expression of functional α-DG in LCMV infected cells. Detection of viral antigens (A) and β-DG (B) in total cell protein: Triplicate samples of A549 cells were infected with LCMV cl-13 (MOI = 0.1) or mock infected. After 4 h, cells were infected with an AdV expressing LARGE (AdV-LARGE), a control AdV-LacZ, or no AdV. Forty-eight hours after infection, total protein was extracted and probed in Western blot with specific antibodies to LCMV GP and NP as described in Figure 4A and β-DG and α-tubulin as described in Figure 1B. The positions of GPC, mature GP2, NP, and α-tubulin are indicated. (C) Overexpression of LARGE restores the expression of functional α-DG in LCMV-infected cells: A549 cells were infected as described in A. After total 48 h, cell surface expression of glycosylated α-DG was assessed by flow cytometry by using mAb IIH6 as in Figure 1D. In histograms, the y-axis represents cell numbers, and the x-axis represents PE fluorescence intensity. Solid line, primary and secondary antibody; broken line, secondary antibody only.