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. 2007 Nov;18(11):4493–4507. doi: 10.1091/mbc.E07-04-0374

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© 2007 by The American Society for Cell Biology

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Figure 8. — DG is required for the association of the viral GP with LARGE. (A) Absence of DG in DG (−/−) cells: Total membrane proteins extracted from DG (+/−) and DG (−/−) cells were probed with mAb IIH6 to glycosylated α-DG (anti-α-DG) and an mAb to β-DG (anti-β-DG). (B) CoIP of LFVGP with LARGE depends on DG: DG (+/−) and DG (−/−) ES cells were cotransfected with LFVGP-flag or Junin GP-flag (JUNGP-flag). After 48 h, IP with flag resin or control matrix was performed as described in Figure 7G. Immunocomplexes were probed with polyclonal antibody to flag (anti-flag), biotinylated mAb 8D5 to β-DG (anti-β-DG), and polyclonal antibody to myc-tag (anti-myc). Positive control lanes (+) correspond to samples of total cell lysates to verify the presence of the indicated proteins.