Figure 6.

GLR-1 accumulation in rab-10 mutants is cholesterol-dependent. Wild-type animals grown on cholesterol-depleted media were categorized as no phenotype (most GLR-1 puncta 0.4–0.8 μm in diameter) (A) or medium phenotype (mostly small GLR-1 puncta, with some GLR-1 puncta 0.8–1.2 μm in diameter; arrowheads) (C). Mutants for rab-10, when grown on cholesterol-depleted media, were similarly categorized as either no phenotype (B), medium phenotype (D), or strong phenotype (E) (contain large GLR-1 accretions >1.5 μm in length; arrows). (F) The frequency of adult animals with the indicated phenotype is plotted for each given genotype and experimental condition (plus or minus cholesterol in growth media). Animals were categorized as described above (no phenotype, white bars; medium phenotype, gray bars; or strong phenotype, black bars). Categorization was scored blindly with respect to genotype. Animals from six separate trials were examined; trial-to-trial variation was minimal. The plot contains data pooled from all six trials. n = 150–200 animals for each genotype. ***p < 0.0001 by Mann–Whitney U test.