Figure 3.
A GFP-Atg18-ALP fusion restores wild-type vacuole morphology in a vac14Δ strain. (A) Fluorescence microscopy comparison of GFP-Atg18 and GFP-Atg18-ALP fusion protein localization and the resulting vacuole morphology in a vac14Δ/atg18Δ strain. Bars, 4 μm. (B) GFP-Atg18-ALP is defective in the cytoplasm-to-vacuole and macroautophagy pathways. APe1 was immunoprecipitated from whole cell lysates of cells metabolically labeled with [35S]methionine (chase time is 2 h for all samples) in the presence or absence of rapamycin. Proteolytic maturation of APe1 was analyzed by SDS-PAGE.