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. 2007 Nov;18(11):4625–4636. doi: 10.1091/mbc.E07-02-0191

Figure 2.

Figure 2.

The tail domain of Myo1B is sufficient for localization. (A) Middle midgut actin visualized with Alexa 568 phalloidin. (B) GFP Myo1B Tail visualized with anti-GFP. (C) Overlay of A and B showing that the tail domain of Myo1B is sufficient for localization to the BB domain of the midgut enterocyte. (D) Western blot probed with anti-GFP to verify expression of GFP Myo1B Tail. Lane 1, WT; lane 2, tail-expressing line. (E) Western blot probed with anti-Myo1B to verify expression of GFP Myo1B tail. Lane 1, WT; lane 2, tail-expressing line. Bar, 20 μm (A–C).