Figure 5.

Loss of Myo1B disrupts BB structure in the larval gut. (A) Low-magnification TEM of the anterior midgut shows that Myo1B heterozygote control has ordered, normal looking MV. (B) Low-magnification TEM of Myo1B mutant anterior midgut. MV are disorganized in appearance, and they are shedding membrane into the lumen of the gut. (C) Low-magnification TEM of P{Myo1B} rescue line anterior midgut. MV are ordered and normal in appearance. (D) High-magnification TEM of Myo1B heterozygote control. MV are densely arrayed and have a uniform morphology. (E) High-magnification TEM of Myo1B mutant anterior midgut; note numerous herniations on MV, vesiculation of MV tips, and general disorganized appearance. (F) High-magnification TEM of P{Myo1B} rescue line. Proper MV ultrastructure is restored by Myo1B expression. Bar, 2 μm (A–C) and 500 nm (D–F).