Loss of Myo1B affects the localization of both singed and Dmoesin in the larval gut. (A) Singed and Dmoesin are expressed at comparable levels in Myo1B heterozygote and mutant whole gut homogenates as compared by Western blot. (B) Loss of Myo1B disrupts singed localization in the proventriculus. Apical BB staining seen in the heterozygote is completely lost in the mutant. (C) Loss of Myo1B disrupts singed localization in cuprophilic cells. The strong staining seen surrounding the cuprophilic cell invagination in the heterozygote is greatly reduced in the Myo1B mutant. (D) Loss of Myo1B reduces the amount of Dmoesin localized to the proventriculus. (E) Loss of Myo1B reduces the amount of Dmoesin that is localized to the apical BB of the anterior midgut. Bar, 20 μm (B), 10 μm (C), 30 μm (D), and 15 μm (E). lu, midgut lumen.