Figure 2.

Dynamin-dependent internalization of VEGFR-2 is required for Akt activation and NO release. (A) K44A-dynamin expression in endothelial cells inhibits Akt activation. Adenovirus-infected BAECs were stimulated with VEGF (40 ng/ml) for the indicated time. Cell lysates were separated by SDS-PAGE and immunoblotted using the indicated antibodies. (B) K44A-dynamin inhibits VEGFR-2–stimulated NO release. COS-7 cells were transfected with VEGFR-2, eNOS, HA-dynamin (HA-dyn), and HA-K44A-dynamin (HA-K44A-dyn) as indicated. Forty-eight hours after transfection, cell culture medium was processed for the measurement of NO. *p < 0.05 versus cells expressing HA-dyn, ANOVA followed by Bonferroni.