Figure 1.

Association of Exo70 with the plasma membrane (PM) in HeLa cells. (A) GFP-Exo70 was detected at the PM. HeLa cells were transfected with GFP-tagged Exo70, fixed, and observed using a confocal microscope. Serial optical sections in the x-z and y-z planes were taken. (B) Various truncates of Exo70 were tested for their localization at the PM. HeLa cells were transfected with GFP-tagged Exo70 and Exo70 truncates as diagramed. The cells were fixed, stained, and observed under microscope. Full-length and the C-terminal fragments of Exo70 containing domain D (aa 558-653) are associated with the PM. +, membrane association.