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. 2007 Nov;18(11):4483–4492. doi: 10.1091/mbc.E07-05-0461

Figure 3.

Figure 3.

Mutations at Exo70 C-terminus abolish the association between Exo70 and the PM. (A) Sequence alignment between the C-termini (domain D) of yeast Exo70 and rat Exo70. The mutated residues in the exo70-1 mutant were marked in grey. (B) The localization of the exo70 mutant, exo70-1, in HeLa cells. HeLa cells were transfected with GFP-tagged wild-type Exo70 as a control (left) and exo70-1 (right). The exo70-1 mutant failed to associate with the PM. (C) Membrane fractionation was performed to examine the localization of GFP-Exo70 and GFP-exo70-1 in HeLa cells. Equal amounts of proteins from each fraction of the cells expressing Exo70 and exo70-1 were loaded on SDS-PAGE. The total proteins in each lane were detected by SYPRORed staining. Exo70 was detected by Western blot. LDM, low-density microsomal membranes, mostly the Golgi fraction; HDM, high-density microsomal membranes, mostly the ER fraction.