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Mutations at the C-terminus of Exo70 affect its interaction with phospholipids. (A) GST-tagged Exo70 and exo70-1 (0.15 μM) were used in the lipid cosedimentation assay. The binding of exo70-1 to 5% PI(4,5)P2 was abolished, whereas its interaction with 60% PS was reduced. (B) Binding curves of Exo70 and exo70-1 to LUVs containing various phospholipids.
