Table 3.
Effect of fascin depletion on the development of primary and metastatic xenograft tumors in nude mice
| a. Mean tumor volume at day 32 | ||
|---|---|---|
| Tumor volume (mm3) ± SEM | ||
| Pa −Dox (n = 4) | 1030.4 ± 506.2 | |
| Pa +Dox (n = 4) | 1151.9 ± 331.7 | |
| F11 −Dox (n = 4) | 1181.3 ± 873.7 | |
| F11 +Dox (n = 4) | 227.7 ± 186.4 | |
| b. Incidence of primary and metastatic tumors from SW480Pa and F11 cells | ||
| % Spleen tumor (n) | % Metastasis (n) | |
| Pa −Dox (n = 5) | 80.0 (4/5) | 100 (5/5) |
| Pa +Dox (n = 3) | 66.7 (2/3) | 100 (3/3) |
| F11 −Dox (n = 4) | 100 (4/4) | 100 (4/4) |
| F11 +Dox (n = 6) | 16.7 (1/6) | 50.0 (3/6) |
| c. Incidence of primary and metastatic tumors from GFP- or XtFascin-rescued F11 cells | ||
| % Spleen tumor (n) | % Metastasis (n) | |
| GFP (n = 6) | 16.7 (1/6) | 0 (0/6) |
| WT (n = 6) | 50.0 (3/6) | 33.3 (2/6) |
| S33A (n = 6) | 33.3 (2/6) | 16.7 (1/6) |
| S33D (n = 6) | 33.3 (2/6) | 0 (0/6) |
Experimental conditions and analyses were as described in Materials and Methods. In a and b, the indicated groups of mice were given 2 mg/ml doxycycline in the drinking water; in c, all mice were given doxycycline.