Figure 6.

Expression of Rab27b and Rab27a in the pituitary. (A) Whole-mount LacZ staining of the pituitary from Rab27b^+/− mice (left panels). Immunohistochemistry with either anti-Rab27b or anti-β-galactosidase antibody in Rab27b^+/+ pituitary (center panels) and Rab27b^−/− pituitary tissues (right panels). ant, anterior lobe; int, intermediate lobe; and post, posterior lobe. Bar, 20 μm. (B) The amounts of Rab27a and Rab27b in lysates of anterior and intermediate-posterior pituitary were determined by immunoblotting with isoform-specific antibodies, using known amounts of GST-Rab27a and GST-Rab27b proteins as standards. (C) The expression levels of endogenous Rab27a and Rab27b in each lobe, which were estimated from the intensities of the immunoblot signals, are shown as a percentage of the total extracted proteins. (D) Thirty micrograms of proteins from the anterior or intermediate-posterior pituitary from Rab27a^ash/ashRab27b^−/− double mutant mice were electrophoresed and immunoblotted with the indicated antibodies.