Figure 2.

In vitro translation of spinophilin or arrestin and pulldown with a GST construct incorporating the large cytoplasmic loop of the Na,K-ATPase α-subunit. Flag-tagged spinophilin and arrestin proteins were prepared by in vitro translation, and GST pulldown was performed with GST alone or GST plus the large cytoplasmic loop of the Na⁺,K⁺-ATPase. Spinophilin (A) and arrestin (B) were detected by Western blot with anti-flag antibody. Both spinophilin and arrestin directly associate with the large cytoplasmic loop of the Na,K-ATPase α-subunit. Typical results from one of three experiments are shown.