Figure 4.
Human telomeric DNA is present at the ends of chromosomes in haploid strains expressing the tlc1-human allele. A Southern blot of XhoI-digested yeast genomic DNA was hybridized first with an oligonucleotide probe specific for yeast telomeric DNA, (TGTGGG)4, stripped, and then reprobed with an oligonucleotide probe specific for human telomeric DNA, (TTAGGG)4. DNA loading varied less than 3-fold between lanes, as determined by rehybridization with a probe for actin and quantitation by using a PhosphorImager. Pop-out, diploid strain C6.1, which has one copy each of the wild-type TLC1 and the tlc1-human alleles; 7A to 8D, haploid strains from the dissection of two tetrads of C6.1.