Figure 4.

Mating reaction of Δuka1 Δubc1 double mutants. The following strains were co-spotted onto mating medium and incubated for 2 days: (A) wild-type strains, 518 (a2 b2) × 521 (a1 b1); (B) Δuka1 single mutants, 001#44(a2 b2 Δuka1-1) × 002#6 (a1 b1 Δuka1-1); (C) ubc1 single mutants, 0505 (a2 b2 Δubc1-4) × 521d (a1 b1 ubc1-2); (D) uka1 ubc1 double mutants, 001#9 (a2 b2 Δuka1-1 Δubc1-3) × 002#86 (a1 b1 Δuka1-1 Δubc1-3). The white mycelium indicative of the formation of dikaryotic hyphae is absent for the ubc1 single mutants; however, a weak mating reaction is observed with the Δuka1 Δubc1 double mutants.