Table 2.

Virulence of mutants defective in genes encoding subunits of PKA

Strains inoculated	No. of plants inoculated	No. of plants with tumors	% of plants with tumors
518 × 521	15	13	87
Δuka1-1 × uka1	46	42	91
Δuka1-1 × Δuka1-1	40	37	93
adr1-1 × adr1	65	8	12
adr1-1 × adr1-1	51	0	0
adr1-1 Δuka1-1 × adr1 uka1	95	2	2
adr1-1 Δuka1-1 × adr1-1 Δuka1-1	95	0	0
d132	63	32	51
adr1-1/adr1	167	82	49
adr1-1/adr1-1	179	0	0
Δubc1-3/ubc1	22	12	55
ubc1-2/ubc1-2	20	0	0
Δubc1-3/ubc1-1	83	0	0

The data for each combination of mutant or diploid strains are pooled from two to three inoculation experiments. The data for heterozygous crosses are pooled from experiments in which the phenotype of the relevant mutations was tested in each of the two strain backgrounds (i.e., 518 and 521) when paired with a compatible wild-type strain. At least two independent transformants (Table 1) were used for each combination of mutant haploid strains or mutant diploid strains, except for the Δubc1-3/ubc1 diploid strain, where data are presented for only one transformant (UD12). Note that diploid strains such as d132 are known to be less virulent than mixtures of compatible haploid strains (10).