Figure 1.

Inhibition of liver metastasis of B16 melanoma cells by in vivo treatment with α-GalCer. (A) Melanoma antigens in metastasized livers measured by RIA using ¹²⁵I-labeled M2590 mAb. B16 melanoma cells (3 × 10⁶ per mouse) were inoculated in the spleen of wild-type (Jα281^+/+), Vα14 NKT-deficient (Jα281^−/−), and Vα14 NKT (RAG^−/−/Vα14tg Vβ8.2tg) mice, followed by intraperitoneal injection with either α-GalCer (100 μg/kg) or vehicle on days 1, 5, and 9. The mice were sacrificed and examined on day 14. Each group consisted of three mice. The data are expressed as a mean cpm value of triplicate samples with standard errors. (B) Photographic views of metastasized livers from mice treated with α-GalCer or vehicle.