Table 1.
Fraction of VDJδ1 sequences among RT–PCR-amplified V-Jα transcripts
| Lane no. | Vδ8 RT–PCR subclones
|
Vδ4 RT–PCR subclones
|
||||||
|---|---|---|---|---|---|---|---|---|
| 11 | 12 | 13 | 15 | 11 | 12 | 13 | 15 | |
| Sample | B6 total | B6 DN | δ−/− 1 | δ−/− 2 | B6 total | B6 DN | δ−/− 1 | δ−/− 2 |
| Cα+ | 57 | 71 (90) | 104 | 60 | 156 (165) | 157 (164) | 160 (167) | 233 (233) |
| Jδ1+ | 0 | 25 (44) | 2 | 0 | 3 (12) | 3 (10) | 21 (28) | 111 (111) |
| Ratio, % | <1.8 | 35 | 1.9 | <1.7 | 1.9 | 1.9 | 13 | 48 |
Vδ8-Cα and Vδ4-Cα cDNA sequences from one wild-type (B6 total and B6 DN) and two independent TCRδ deficient (δ−/−) mice were PCR-amplified, subcloned, and screened separately with Cα- and Jδ1-gene-specific oligonucleotide probes under high-stringency conditions. The samples correspond to those shown in Fig. 4, lanes 11, 12, 13 and 15. The number of unique positive, the total number of positive (in parentheses), and the ratio of unique Jδ1+ to Cα+ clones, expressed in percentage, are shown. The number of unique Jδ1+ clones was extrapolated from direct sequencing of 10 B6 total, 16 B6 DN (for Vδ4 and Vδ8 products), and 12 δ−/− 1 and 12 δ−/− 2 individual clones.