Figure 1.

Comparison of AsNOS (GenBank accession no. AF053344) and DNOS (9) deduced amino acid sequences. The first AsNOS amino acid is the putative initiation methionine; alignment with DNOS begins at D₈₁. Presumed cofactor-binding domains for heme, calmodulin (CaM), FMN, FAD pyrophosphate (FAD PPi), FAD isoalloxazine (FAD Iso), NADPH ribose, NADPH adenine, and NADPH are double-underlined (9). Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.