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The antagonist peptide NP34 rescues F5 thymocytes from negative selection in F5/NP FTOC. Fetal thymic lobes were isolated from day 15 F5/NP68/Rag-1−/− (F5/NP) and F5/Rag-1−/− (F5) embryos and were cultured alone or in the presence of 10 μM NP34 or 10 μM GAG peptides. Thymocytes were harvested at day 10 of culture and stained for CD4 and CD8 molecules. The percentage of cells in each quadrant region is indicated in the dot plots.
