Figure 4.

NP34 accelerates the production of mature thymocytes in F5 FTOC. F5 FTOC were cultured in the presence of 10 μM NP34 (circles and open bars) or 10 μM GAG (triangles and shaded bars) peptides for 3, 6, 8, or 10 days. (A) Thymocytes were harvested at each time point and stained with anti-CD4, anti-CD8, and anti-HSA mAbs. Each symbol represents absolute number of CD8⁺CD4⁻HSA^lo thymocytes generated from an individual FTOC, and bars represent the mean of these numbers. Similar data were obtained from two independent time course experiments. (B) Thymocytes harvested from NP34-treated (Left) and GAG-treated (Right) FTOC at days 6, 8, and 10 were stained with anti-CD4, anti-CD8, and 7AAD. Histograms show the 7AAD profiles of the CD8 SP thymocytes included in the gate shown in the dot plots. The percentage of cycling thymocytes is indicated above the marker in the histograms.