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. 1998 May 12;95(10):5712–5717. doi: 10.1073/pnas.95.10.5712

Figure 5.

Figure 5

Expression of FIM and of the fusion transcripts. (a) Northern blot analysis of the expression of FIM. Northern blots with indicated human adult poly(A)+ RNAs were hybridized with the FIM 27.1a probe. β-actin was used as control. (b) RT-PCR products obtained from SCID t(8;13) and normal breast (Upper and Lower Right, respectively) RNAs using FGFR1 and FIM primers located near the translocation, without (four first lanes) or with (five last lanes) oligonucleotide competition. (Right) Primers used were: lane 1, FA and F9; lane 2, X1 and 1R; lane 3, FA and 1R; lane 4, X1 and F9; lane 5, FA, F9, and 1R; lane 6, FA, F9, and X1; lane 7, X1, 1R, and FA; lane 8, F9, X1, and 1R; and lane 9, FA, F9, X1, and 1R. The two variant products of the wild-type FGFR1 gene of 180 and 186 bp (FA and F9 primers) are seen in lanes 1, 5, 6, and 9. β-actin primers were used to estimate the efficiency of the PCR reactions (Left). The respective chromosomal positions and the transcripts identified in each reaction are indicated at the top and on the right, respectively.