Skip to main content
. 1998 May 12;95(10):5712–5717. doi: 10.1073/pnas.95.10.5712

Figure 7.

Figure 7

Autokinase activity and phosphorylation on tyrosine of FIM/FGFR1. The FGFR1 overexpressing cell line (NFlg26) and NIH 3T3 cells transiently transfected with either FIM/FGFR1 cDNA (pCHIM) or the empty vector (pcDNA3) were treated with (+) or without (−) FGF1. Immunoprecipitates using anti-C-FGFR1 antibody were analyzed for autokinase activity (Left) (autoradiography of 15 hr for NFlg26 and 45 hr for pCHIM and pcDNA3), phosphorylation on tyrosine after Western blot with an anti-phosphotyrosine antibody (4G10) and expression level by Western blot with anti-C-FGFR1 antibody (Right). The position of molecular mass standards (in kDa) is indicated.