Abstract
1. The in vitro immunological release of histamine and slow reacting substance of anaphylaxis (SRS-A) from actively sensitized guinea-pig lung fragments was greatly reduced when the animals were maintained on an ascorbic acid-deficient diet. Excessive dietary levels of ascorbic acid did not increase mediator release above normal levels. 2. Restoration of ascorbic acid in the diet of scorbutic guinea-pigs restored in vitro immunological histamine to normal levels. 3. Variation in dietary levels of ascorbic acid had no effect on lung histamine content. 4. The lung ascorbic acid content was proportional to the dietary intake. Approximately 60% of the total lung ascorbic acid was removed by the process of chopping and washing of the tissue. This relationship was independent of dietary intake. 5. The results indicate that the immunological release of mediators of inflammation from guinea-pig lung is dependent on adequate endogenous levels of ascorbic acid.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DAWSON W., WEST G. B. THE INFLUENCE OF ASCORBIC ACID ON HISTAMINE METABOLISM IN GUINEA-PIGS. Br J Pharmacol Chemother. 1965 Jun;24:725–734. doi: 10.1111/j.1476-5381.1965.tb01628.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dawson W., West G. B. The nature of the antagonism of bronchospasm in the guinea-pig by ascorbic acid. J Pharm Pharmacol. 1965 Sep;17(9):595–596. doi: 10.1111/j.2042-7158.1965.tb07729.x. [DOI] [PubMed] [Google Scholar]
- GUIRGIS H. M. ANTI-ANAPHYLACTIC EFFECT OF VITAMIN C IN THE GUINEA-PIG. J Pharm Pharmacol. 1965 Jun;17:387–387. doi: 10.1111/j.2042-7158.1965.tb07690.x. [DOI] [PubMed] [Google Scholar]
- Hitchcock M. Effect of inhibitors of prostaglandin synthesis and prostaglandins E2 and F2alpha on the immunologic release of mediators of inflammation from actively sensitized guinea-pig lung. J Pharmacol Exp Ther. 1978 Nov;207(2):630–640. [PubMed] [Google Scholar]
- Kordansky D. W., Rosenthal R. R., Norman P. S. The effect of vitamin C on antigen-induced bronchospasm. J Allergy Clin Immunol. 1979 Jan;63(1):61–64. doi: 10.1016/0091-6749(79)90163-5. [DOI] [PubMed] [Google Scholar]
- Parkes D. G., Eling T. E. Characterization of prostaglandin synthetase in guinea pig lung. Isolation of a new prostaglandin derivative from arachidonic acid. Biochemistry. 1974 Jun 4;13(12):2598–2604. doi: 10.1021/bi00709a020. [DOI] [PubMed] [Google Scholar]
- SHORE P. A., BURKHALTER A., COHN V. H., Jr A method for the fluorometric assay of histamine in tissues. J Pharmacol Exp Ther. 1959 Nov;127:182–186. [PubMed] [Google Scholar]
- Watanabe-Kohno S., Parker C. W. Role of arachidonic acid in the biosynthesis of slow reacting substance of anaphylaxis (SRS-A) from sensitized guinea pig lung fragments: evidence that SRS-A is very similar or identical structurally to nonimmunologically induced forms of SRS. J Immunol. 1980 Aug;125(2):946–955. [PubMed] [Google Scholar]
- Willis R. J., Kratzing C. C. Ascorbic acid in rat lung. Biochem Biophys Res Commun. 1974 Aug 19;59(4):1250–1252. doi: 10.1016/0006-291x(74)90448-3. [DOI] [PubMed] [Google Scholar]
- Zuskin E., Lewis A. J., Bouhuys A. Inhibition of histamine-induced airway constriction by ascorbic acid. J Allergy Clin Immunol. 1973 Apr;51(4):218–226. doi: 10.1016/0091-6749(73)90141-3. [DOI] [PubMed] [Google Scholar]