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. 2007 May 30;14(8):959–968. doi: 10.1128/CVI.00123-07

FIG. 1.

FIG. 1.

BKV agnoprotein expression in kidney tubular epithelial cells. (A) Indirect immunofluorescence of primary kidney proximal tubular epithelial cell culture infected with BKV strain Dunlop and stained at 48 h postinfection with rabbit anti-BKV agnoprotein serum (green) and anti-SV40 LT Pab416 (red). Arrows indicate cells with LT antigen (cell 1), LT antigen plus a little agnoprotein (cell 2), and LT antigen plus abundant agnoprotein (cell 3) (note that there are no cells with agnoprotein alone). (B) Immunohistochemistry of renal allograft biopsy specimen stained with anti-BKV agnoprotein serum (brown). Magnification, ×100. (C) Immunohistochemistry of renal allograft biopsy specimen stained with anti-BKV agnoprotein serum (brown). Magnification, ×400. (D) Immunohistochemistry of renal allograft biopsy specimen double stained with anti-BKV agnoprotein serum (red) and anti-SV40 LT Pab416 (brown). Magnification, ×1,000.