Distribution of individual results of five commercially available ELISAs (Chagas III, ELISAcruzi, Chagatek, Chagatest Rec v3.0, and Pathozyme) and one in-house ELISA (ELISA-IMT) for diagnosis of Chagas' disease. The first five groups of data in each graph are results, from study 1, for 53 Brazilian individuals with T. cruzi infections (Ch), 45 healthy individuals (H), 21 patients infected with Leishmania spp. (L), 23 individuals infected with Trypanosoma rangeli (Tr), and 45 patients with other infections (OI). The last group of data in each graph (Study 2) corresponds to 16 TESA-blot-positive Panamanian cases. The horizontal line within each data group is the arithmetic mean. Each data point was calculated as the ratio of the OD to the cutoff. The horizontal line at 1.0 unit represents the cutoff.