TABLE 1.
Numbers and percentages of positive cases obtained by six ELISAs for Chagas' disease diagnosis using the TESA blot as a reference test
| Status of patients | No. of cases | No. (%) of positive cases by:
|
||||||
|---|---|---|---|---|---|---|---|---|
| TESA blot (IMT)a | ELISA-IMTa,b | Chagas III (BIOSChile)b | ELISAcruzi (bioMérieux Brasil SA)b | Chagatek (bioMérieux Brasil SA)b | Chagatest Rec v3.0 (Wiener)c | Pathozyme Chagas (Omega)c | ||
| Study 1 | 187 | |||||||
| Chagas' disease patients | 53 | 53 (100) | 53 (100) | 53 (100) | 53 (100) | 53 (100) | 53 (100) | 53 (100) |
| Subjects without Chagas' disease | 134 | |||||||
| Healthy | 45 | 0 | 0 | 0 | 1 (2.22) | 0 | 0 | 0 |
| VLd | 13 | 0 | 12 (92.31) | 12 (92.31) | 11 (84.60) | 11 (84.60) | 0 | 0 |
| CLe | 8 | 0 | 6 (75.00) | 1 (12.50) | 7 (87.50) | 6 (75.00) | 0 | 0 |
| T. rangeli | 23 | 0 | 2 (8.69) | 0 | 0 | 0 | 0 | 0 |
| Other infections | 45 | 0 | 0 | 0 | 4 (8.88) | 0 | 0 | 0 |
| Study 2 (Panamanians) | 120 | 16 (13.33) | 18 (15.00) | 16 (13.33) | 18 (15.00) | 18 (15.00) | 13 (10.83) | 12 (10.00) |
| TESA blot positive | 16 | 16 (100) | 16 (100) | 15 (93.75) | 15 (93.75) | 16 (100) | 13 (81.25) | 12 (75.00) |
| TESA blot negative | 104 | 0 | 2 (1.92) | 1 (0.96) | 3 (2.88) | 2 (1.92) | 0 | 0 |
a
Test prepared at the IMT.
b
ELISA prepared with T. cruzi epimastigote antigens.
c
ELISA prepared with T. cruzi recombinant proteins.
d
VL, visceral leishmaniasis.
e
CL, cutaneous leishmaniasis.