TABLE 2.
Sensitivities and specificities of seven assays to detect Trypanosoma cruzi antibodies
| Test | Study 1 (n = 187)
|
Study 2 (n = 120)
|
|||
|---|---|---|---|---|---|
| Sensitivitya (n = 53) | Specificitya,b
|
Sensitivity (n = 16) | Specificity (n = 104) | ||
| Including leishmaniasis cases (n = 134) | Excluding leishmaniasis cases (n = 113) | ||||
| TESA blot (IMT) | 100 (94.5-100) | 100 (97.8-100) | 100 (97.4-100) | 100 (82.9-100) | 100 (97.2-100) |
| ELISA-IMTc | 100 (94.5-100) | 85.07 (78.3-90.4) | 98.23 (94.3-99.7) | 100 (82.9-100) | 98.08 (93.8-99.7) |
| Chagas III (BIOSChile)c | 100 (94.5-100) | 90.30 (84.3-94.5) | 100 (97.4-100) | 93.75 (72.8-99.7) | 99.04 (95.4-100) |
| ELISAcruzi (bioMérieux Brasil)c | 100 (94.5-100) | 82.84 (75.7-88.5) | 95.57 (90.5-98.4) | 93.75 (72.8-99.7) | 97.12 (92.4-99.3) |
| Chagatek (bioMérieux Brasil)c | 100 (94.5-100) | 87.31 (80.9-92.2) | 100 (97.4-100) | 100 (82.9-100) | 98.08 (93.8-99.7) |
| Chagatest Rec v3.0 (Wiener)d | 100 (94.5-100) | 100 (97.8-100) | 100 (97.4-100) | 81.25 (57.0-95.0) | 100 (97.2-100) |
| Pathozyme Chagas (Omega)d | 100 (94.5-100) | 100 (97.8-100) | 100 (97.4-100) | 75 (50.1-91.5) | 100 (97.2-100) |
a
Both sensitivity and specificity are given as percentages, with 95% CIs in parentheses. Confidence intervals were calculated using the mid-P 95% confidence interval (95% CI) with Epi Info 6.0 software.
b
Determined with sera from non-Chagas' disease patients.
c
ELISA prepared with T. cruzi epimastigote antigens.
d
ELISA prepared with T. cruzi recombinant proteins.