FIG. 5.
Immunization with SA85-1.1 protein during T. cruzi infection enhances anti-SA85-1.1 antibody responses but does not alter tissue inflammation. C57BL/6 mice were inoculated with 1 × 105 trypomastigotes, immunized with SA85-1.1 or OVA in complete Freund's adjuvant on day 28 of the infection, and 14 days and 28 days later, immunized with the same proteins again in incomplete Freund's adjuvant. (A) Sera were collected 40 and 62 days after the initial immunization (on days 68 and 90 of infection), and SA85-1.1 IgG, IgG2a, and IgG1 end-point titers were determined. Results are expressed as means and SE for at least four mice per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (B) Skeletal muscles were collected on day 42 and day 69 of infection, sectioned, and H&E stained, and inflammatory scores were determined. Results are expressed as means and SE for four muscles per group.