Competition for VlsE binding with the C6 peptide. Individual serum samples were incubated with increasing concentrations of peptide for 1 h prior to their addition to VlsE antigen-coated 96-well plates. Shown are OD450 values obtained for each sample. Error bars represent the standard deviations of the means of triplicate determinations. The levels of inhibition with 1,000 ng C6 peptide/well added were as follows: NA6, 69%; NA8, 17%; NA10, 95%; NA11, 66%; NA12, 53%; NA13, 39%; NA15, 10%; and NA20, 15%.