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. 2007 Jun 13;14(8):931–936. doi: 10.1128/CVI.00075-07

TABLE 2.

ELISA values for Lyme disease patient serum samples tested for IgG reactivity to VlsE and VlsE-derived peptides

Patient Diagnosisa ELISA result (value) for diagnosisb Immunoblot resultc
IgG ELISA result (value)b,d
IgM IgG Ct N1 N2 N3 C2 C4 C6 VlsE
NA6 LA P (6.90) N P P (1.602) N (0.146) P (0.754) N (0.107) N (0.119) N (0.097) P (1.353) P (0.275)
NA7 Clinical P (1.93) N P N (0.157) N (0.119) N (0.144) N (0.102) N (0.069) N (0.105) P (0.224) N (0.081)
NA8 Clinical P (4.41) P P P (2.057) P (0.803) N (0.097) N (0.160) N (0.103) N (0.106) P (1.031) P (0.319)
NA9 EM, LA P (5.75) N P N (0.091) N (0.127) N (0.0890) N (0.087) N (0.152) N (0.144) P (0.293) N (0.090)
NA10 LA P (7.48) N P P (1.026) N (0.207) P (2.105) N (0.168) N (0.241) N (0.154) P (2.263) P (1.450)
NA11 Clinical P (3.51) N P P (1.007) N (0.321) P (0.3) N (0.099) N (0.135) N (0.214) P (0.770) P (0.440)
NA12 EM, clinical P (4.33) P P P (1.800) N (0.079) N (0.076) N (0.117) N (0.096) N (0.093) P (0.899) P (0.639)
NA13 LA P (8.45) P P P (2.429) P (0.433) P (1.268) P (1.495) P (0.270) P (0.271) P (1.428) P (1.605)
NA14 LA P (5.49) N P N (0.174) N (0.305) P (0.208) N (0.175) N (0.239) P (0.258) P (0.454) P (0.192)
NA15 EM, clinical P (5.98) P P P (2.522) N (0.331) P (0.191) P (2.086) N (0.15) N (0.13) P (2.101) P (2.136)
NA16 LA P (8.18) N P P (0.872) N (0.123) P (0.690) N (0.061) N (0.105) N (0.113) P (0.591) P (0.207)
NA17 LA P (3.77) N P N (0.077) N (0.152) N (0.123) N (0.082) N (0.123) N (0.111) P (1.025) P (0.220)
NA20 CSF C+ P (5.82) P P P (2.241) P (0.602) P (0.802) P (0.275) N (0.118) N (0.116) P (1.458) P (0.853)
NA22 CSF C+ P (1.24) N P N (0.133) N (0.191) N (0.094) N (0.066) N (0.106) N (0.135) P (0.390) N (0.090)
NA18 EM, C+ P (2.58) N P N (0.062) N (0.233) N (0.071) N (0.064) N (0.103) N (0.088) P (0.684) N (0.123)
NA19 EM, C+ P (1.89) P P N (0.092) N (0.213) N (0.110) N (0.087) P (0.316) N (0.139) P (0.609) N (0.112)
NA21 EM, C+ P (3.87) P P P (1.661) E (0.420) N (0.153) P (0.225) N (0.185) N (0.171) P (1.464) P (0.195)
NA23 EM, C+ P (4.77) P P N (0.180) P (0.486) N (0.101) N (0.083) N (0.147) N (0.176) P (2.543) P (1.045)
NA24 EM, C+ P (3.91) P N N (0.306) N (0.256) N (0.132) N (0.127) N (0.190) N (0.158) P (1.993) P (0.160)
NA25 EM, C+ P (3.49) P N N (0.154) N (0.152) N (0.103) N (0.082) N (0.149) N (0.126) P (1.309) N (0.106)
NA26 EM, C+ P (2.80) P N P (0.935) P (1.073) P (0.507) P (0.483) P (0.485) P (0.435) P (1.025) N (0.150)
NA27 EM, C+ P (1.45) P N N (0.121) P (0.460) N (0.120) N (0.096) P (0.302) N (0.123) P (0.415) N (0.099)
NA28 EM, C+ P (2.27) P N P (0.665) N (0.281) N (0.135) N (0.109) N (0.178) N (0.168) P (1.054) N (0.137)
NA29 EM, C+ N (0.53) P N N (0.109) N (0.131) N (0.094) N (0.073) N (0.117) N (0.109) P (0.676) N (0.084)
NA30 EM, C+ P (1.75) N N N (0.129) N (0.186) N (0.107) N (0.075) N (0.113) N (0.104) N (0.202) N (0.106)
NA31 EM, C+ E (0.99) P N N (0.102) N (0.106) N (0.067) N (0.065) N (0.092) N (0.093) N (0.159) N (0.065)
NA32 EM, C+ E (0.95) P N P (0.766) N (0.289) N (0.140) N (0.125) N (0.143) N (0.142) P (0.425) N (0.117)
NA33 EM, C+ P (1.30) N N N (0.226) P (0.547) N (0.150) N (0.144) N (0.202) N (0.187) N (0.238) N (0.126)
NA34 EM, C+ P (1.58) P N N (0.195) N (0.133) N (0.074) N (0.082) N (0.113) N (0.143) P (1.153) N (0.110)
NA35 EM, C+ P (1.59) P N N (0.102) N (0.091) N (0.065) N (0.088) N (0.1) N (0.089) P (0.432) N (0.089)
NA36 Rash, C+ P (1.29) N N N (0.183) N (0.127) N (0.088) N (0.121) N (0.131) N (0.126) N (0.180) N (0.137)
NA37 EM, C+ N (0.18) N N N (0.114) N (0.233) N (0.072) N (0.075) N (0.124) N (0.135) N (0.159) N (0.073)
NA38 EM, C+ N (0.59) N N N (0.407) N (0.357) N (0.153) N (0.130) N (0.173) N (0.167) P (0.637) N (0.119)
NA39 EM, C+ N (0.05) N N N (0.157) N (0.098) N (0.077) N (0.081) N (0.115) N (0.116) N (0.173) N (0.087)
NA40 EM, C+ N (0.57) N N N (0.212) N (0.275) N (0.077) N (0.075) N (0.102) N (0.130) P (0.356) N (0.081)
NA41 EM, C+ N (0.58) N N N (0.167) N (0.186) N (0.111) N (0.129) N (0.194) N (0.183) N (0.318) N (0.133)
NA42 EM, C+ E (0.93) N N N (0.096) N (0.101) N (0.109) N (0.079) N (0.116) N (0.202) N (0.270) N (0.115)
a

C+, culture positive; CSF, cerebrospinal fluid; LA, Lyme arthritis. Clinical diagnoses were made as described in Materials and Methods.

b

P, positive; N, negative; E, equivocal (indeterminate). Bold values (positive) indicate samples with OD values greater than the mean plus 3 standard deviations of the negative control values.

c

Lyme disease Marblot (MarDx).

d

The values for control patients NA1 to NA5 were used to calculate the cutoff value (mean + 3 standard deviations). These were run in tandem on the ELISA plates with patient samples NA6 to NA31 or NA32 to NA42. The means + standard deviations (cutoff values) for these patients were as follows: for patients NA6 to NA31, 0.128 + 0.046 (0.265), 0.1842 + 0.079 (0.421), 0.096 + 0.024 (0.168), 0.116 + 0.025 (0.190), 0.150 + 0.039 (0.267), 0.126 + 0.032 (0.222), 0.115 + 0.036 (0.223), and 0.079 + 0.016 (0.127) for Ct, N1, N2, N3, C2, C4, C6, and VlsE, respectively; and for patients NA32 to NA42, 0.194 + 0.101 (0.497), 0.180 + 0.082 (0.425), 0.097 + 0.026 (0.173), 0.111 + 0.019 (0.168), 0.146 + 0.049 (0.292), 0.136 + 0.042 (0.260), 0.150 + 0.065 (0.344), and 0.085 + 0.025 (0.158) for Ct, N1, N2, N3, C2, C4, C6, and VlsE, respectively.