Figure 4.

Effects of inhibitors of Src, MEK1/2, and NF-κB on up-regulation of MUC2 mRNA induced by P. aeruginosa. HM3 cells were pretreated with PP1 (14 μM, 30 min), PD98059 (37 μM, 30 min), or CAPE (15 μg/ml, 60 min) and then exposed to PAO1 culture supernatant (CS) for 6 hr before harvesting for RNA extraction. RPA was then performed with the MUC2 gene-specific probe HAM1. A cyclophilin probe was included as a control to assess amount of RNA used in each hybridization reaction. Similar results were observed in another MUC2-expressing epithelial cell line, NCIH292.