Figure 5.

Effects of inhibitors of Src, MEK1/2, and NF-κB on up-regulation of MUC2 transcription induced by P. aeruginosa LPS. HM3 cells were transfected with a MUC2 base pair −1,600/−1,300 TK promoter construct. Forty hours later, cells were pretreated with PP1 (14 μM, 30 min), PD98059 (37 μM, 30 min), or CAPE (15 μg/ml, 60 min) and then exposed to P. aeruginosa LPS (15 μg/ml) for 6 hr before harvesting for assessing luciferase activity. Similar results were observed in another MUC2-expressing epithelial cell line, NCIH292.