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. Author manuscript; available in PMC: 2007 Oct 29.
Published in final edited form as: J Biol Chem. 2007 Jul 12;282(37):27100–27114. doi: 10.1074/jbc.M701316200

FIGURE 2. Apoptosis and caspase-3 activation induced by free cholesterol loading of peritoneal macrophages from wild-type and iPLA2β-null mice.

FIGURE 2

A, left column represents control macrophages from wild-type (WT) or iPLA2β-knock-out (KO) mice, and the right column represents macrophages subjected to FCL. In the 2nd and 4th rows, externalization of phosphatidylserine was visualized with Alexa-488-labeled annexin V. Total cells were determined from the light micrographs in the 1st and 3rd rows. B, percent of apoptotic cells was determined from annexin-positive and total cells in six fields of 1000 cells each. C displays macrophage caspase-3 activity. B and C, CON denotes control macrophages, and FCL denotes free cholesterol-loaded macrophages. Mean values ± S.E. are displayed (n = 6). Asterisk denotes p < 0.05 for WT versus KO.