TABLE 2.

PCR primers used in this study

Primer	Sequencea	Annealing temp (°C)	Product size (bp)
LIP8a	AGAGTGATACAGACAAAAAATCAG	59	521
LIP8b	AAGACCATTCAGCATCATGGTG
LIP8-3′-SalI	GTCGACCAACATGATAAAAGATTAAGTAAC	56	382
LIP8-3′-PstI	CTGCAGTACTAGACACTTACAATTTACA
LIP8-5′-KpnI	GGTACCAATTCCGGATACTCATTAGC	55	816
LIP8-5′-SacI	GAGCTCAGTTGTATTCATTTCTATCCAC
LIP8-Re-5′-KpnI	GGTACCATGTTGTTTTTATTATTCTTATTAATTAC	60	1,803
LIP8-Re-3′-NcoI	CCATGGATAGATAAGAAAATCCGGCTCAAC
LIP8-Re-3′-3-SacI	GAGCTCATAGATAAGAAAATCCG
Actin-5′-KpnI	GGTACCATGTCTTTAGAGCCTTCAGG	55	1,455
Actin-3′-KpnI	GGTACCCATACCAGAACCGTTATCG
Actin-5′-3-SacI	GAGCTCATGTCTTTAGAGCCTTCAGG	55	3,252
KO-3-5′	ATATTTTTAATATCCACACTGGC	55	354
KO-3-3′	AACATCTTTGCTATATTTAGGTG
EFB1a	ATTGAACGAATTCTTGGCTGAC	55	554
EFB1b	CATCTTCTTCAACAGCAGCTTG

^aRecognition sites of restriction enzymes are indicated by bold type.