TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Y. enterocolitica strains | ||
| JB580v | ΔyenR (r− m+) Nalr, serogroup O:8 | 32 |
| GHY147 | JB580v, pTP166Kan-damΔ, Nalr Kanr | 22 |
| GHY150 | JB580v, pTP166Kan, Nalr Kanr | 22 |
| GHY174 | JB580v, flhD::lacZYA, Nalr Camr | This study |
| GHY175 | JB580v, flhD::lacZYA, pTP166Kan-damΔ, Nalr, Camr Kanr | This study |
| GHY176 | JB580v, flhD::lacZYA, pTP166Kan, Nalr Camr Kanr | This study |
| GHY287 | JB580v, inv::pEP-inv, Nalr Camr | This study |
| E. coli strains | ||
| DH5α | Φ80dΔ(lacZ)M15 Δ(argF-lac)U169 endA1 recA1 hsdR17(rK− mK+) deoR thi-1 supE44 gyrA96 relA1 | Gibco BRL |
| S17-1λpir | Tpr SmrrecA thi pro hsdR M+ RP4::2-Tc::Mu::Km Tn7λpir lysogen | 39 |
| Plasmids | ||
| pFUSE | Camr, mob+ (RP4), R6K ori (suicide vector) lacZYA | 6 |
| pEP185.2 | Camr, mob+ (RP4), R6K ori (suicide vector) | 32 |
| pTP166Kan | Kanamycin-resistant derivative (Amps) of pTP166 | 22 |
| pTP166Kan-damΔ | dam mutant derivative of pTP166Kan | 22 |
| pFUSE-flhD | flhD promoter fragment in pFUSE | This study |
| pEP-inv | Internal fragment of inv in pEP185.2 | This study |