TABLE 3.

Relative gene expression after overproduction of Dam

Gene, temp (°C)	Rel. expressiona	P valueb	Fold changec
inv, 26	0.181 ± 0.0194	0.0008*	↓5.52
inv, 37	0.573 ± 0.2061	0.023*	↓1.75
ail, 26	0.729 ± 0.0653	0.002*	↓1.37
ail, 37	0.875 ± 0.3599	0.51	↓1.14
pilL, 26	0.363 ± 0.2505	0.014*	↓2.75
pilL, 37	0.902 ± 0.2339	0.519	↓1.11
myfA, 26	0.902 ± 0.1761	0.393	↓1.11
myfA, 37	0.922 ± 0.5869	0.83	↓1.08
flp-1, 26	0.184 ± 0.1070	0.0001*	↓5.43
flp-1, 37	1.080 ± 0.8303	0.811	↑1.08
ddhA, 26	0.680 ± 0.5963	0.32	↓1.47
ddhA, 37	1.325 ± 0.2514	0.088	↑1.33
gne, 26	0.599 ± 0.5557	0.2	↓1.67
gne, 37	1.205 ± 0.3425	0.36	↑1.21
rosA, 26	0.567 ± 0.5211	0.15	↓1.76
rosA, 37	1.120 ± 0.2364	0.43	↑1.12

^aRelative expression. Relative mRNA amounts were determined by qRT-PCR and related to mRNA levels in control cells without overproduction of Dam, set as 1. Values are means ± standard deviations.

^bSignificance of difference (P < 0.05) from level in control cells without overproduction of Dam was calculated by Student's t test and is indicated by an asterisk.

^cn-fold change (↓, downregulation; ↑, upregulation) compared to relative expression in control cells without overproduction of Dam.