FIG. 4.

Effect of TNF-α-expressing adenoviral vector on pulmonary inflammation following C. neoformans infection. The groups are the same as those described in the legend to Fig. 3. Whole-lung leukocytes were prepared and enumerated as described in Materials and Methods, using Wright-Giemsa staining to distinguish neutrophils (Neut), eosinophils (Eos), and mononuclear cells (Mono). Eosinophil numbers were additionally confirmed by flow cytometry (see Fig. 5). One asterisk, P < 0.05 for a comparison with the C.neo group. The leukocyte cell numbers on days 7 and 14 for all groups were significantly different from the numbers for uninfected mice (P < 0.05); the only exception was eosinophils in the C.neo + TNFα Adv group.