FIG. 9.

Effect of TNF-α-expressing adenoviral vector on (A) macrophage expression of YM1 and YM2 and (B) whole-lung expression of macrophage differentiation markers following C. neoformans infection (days 3 and 7). mRNA expression was analyzed by RT-PCR, and the groups are the same as those described in the legend to Fig. 1. (A) Leukocytes were prepared from whole lungs as described in Materials and Methods and enriched by plate adherence. (B) Whole-lung mRNA was isolated as described in Materials and Methods. Each lane represents one of at least three animals per group. AMCase, acidic mammalian chitinase.