FIG. 3.

(A) Spot plate assay showing the sorbitol-remediable 37°C growth defect of the ccr4Δ mutant on YPD agar (left panel) and on Asn salts agar containing 2% dextrose and 1 mg/ml yeast nitrogen base medium (ASN 2%DEX YNB) with (right panel) and without (center panel) 1 M sorbitol as an osmostabilant. The spots contained 5 μl of a cell suspension at an OD₆₀₀ of 1.0 and five 1:5 dilutions. The plates were incubated for 3 days at 37°C. (B) Photomicrographs showing cells of the wild type (top) and the ccr4Δ mutant (bottom) grown on Asn salts agar at 37°C stained with calcofluor white. Scale bars = 5 μm. The arrowheads indicate wild-type calcofluor white accumulation at the bud necks. wt, wild type.