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(A) India ink staining of the wild type and the ccr4Δ mutant grown under capsule-inducing conditions. (B and C) Analysis of capsular permeability by TMRD incorporation (B) and deposition of monoclonal antibody 3C2 Fab fragments (C) assessed 15 and 30 min after staining, respectively. Scale bars = 5 μm. wt, wild type; BF, bright field; CFW, calcofluor white.
