FIG. 5.

Patterns of MBL binding to the wild-type, ccr4Δ, and cap60Δ strains grown to mid-log phase at 30°C in YPD medium, followed by serum opsonization at 37°C. Cells were stained with either anti-MBL monoclonal antibody or anti-c-myc monoclonal antibody as an IgG1 isotype control, followed by an AlexaFluor 488-conjugated goat-anti-mouse IgG secondary antibody (Invitrogen). For the cap60Δ strain, the same cell is shown in two separate focal planes; the top images demonstrate MBL binding to the bud neck, and the bottom images demonstrate MBL binding at the bud scar. Scale bars = 2.5 μm. wt, wild type; BF, bright field; CFW, calcofluor white; mAb, monoclonal antibody.