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. 2007 Jul 16;75(10):4851–4856. doi: 10.1128/IAI.00314-07

FIG. 2.

FIG. 2.

Demonstration of surface expression of bovine CD18 chimeric antigens in 2 of the 13 transductants, B200H and H582B617H, described in the study. Surface expression of chimeric CD18 antigens in the various transductants was confirmed by FACS using anti-CD18-specific MAb BAQ30A. The parent cell line K562 (open trace), which does not express CD18 antigen, was used as a negative control. The expression levels of chimeric CD18 antigens in the other 11 transductants were similar (data not shown). The x axis shows the fluorescence intensity (FL2-H), and the y axis shows cell number (counts); the numbers shown within the panels are the percentages of positive CD18 cells. Results show high levels of expression of CD18 antigen in chimeric transductants (solid traces). Data presented are representative of one of three experiments performed. FL2-H, height for phycoerythrin; M1, marker 1.