FIG. 5.

Immunofluorescence of BFP. HeLa cells were infected with wild-type strain E2348/69, which expresses α₁ bundlin; with wild-type strain RN587/1, which expresses β₆ bundlin; or with corresponding bfpA mutants UMD901 and UMD949, each transformed with plasmid pIL14 encoding an afimbrial adhesin to compensate for poor adherence. Infected cells were examined by immunofluorescence microscopy using affinity-purified antisera against α₁ and β₆ bundlins, as indicated. Corresponding phase-contrast and fluorescence micrographs are shown.